Above 100gml, the ethanolic extract showed 80% scavenging activity, similar to control antioxidant compounds quercetin, rutin and lascorbic acid. Free radical scavenging capacity and antioxidant activity. Pdf antioxidant activity by dpph radical scavenging. The present study was carried out to evaluate the free radical scavenging activity of a poly herbal extract and its cytotoxicity in brl3a cell line by srb assay. A perusal of the publications in the recent past table 1 shows that various research groups have used widely different protocols which differed in the concentration of dpph 22. In vitro antioxidant and free radical scavenging activity. Materials and methods dpph free radical scavenging activity processing of plants for extract preparation. The antioxidant activity was expressed as a percentage of scavenging activity on dpph radical. Free radical scavenging potential of a poly herbal formulation.
Free radical scavenging activities of solutions of the plant extracts and synthetic antioxidant substances used in the study prepared in methanol at concentrations of 50, 100 and 200. Antioxidant potential of the plant extract was measured in. Phytochemical analysis and free radical scavenging activity. Archana cm et al, international journal of pharmaceutical sciences and business management, vol.
Free radical scavenging activity of gossypin and nevadensin. All extracts were able to reduce the stable, purplecoloured radical dpph into yellowcoloured dpphh. Zhenget al salidroside analogues 655 alkylation of diethylmalonate20 with 7 yielded the substituted diethyl benzylmalonate 11w 88%, 11x81%, while subsequent deethoxycarbonylation21 of 11 with sodium chloride in wet dmso lead to 12w in 95% yield and 12x in 92% yield. Radical scavenging activity of extract, fraction and. Free radical scavenging activity of ethanolic extracts from. Evaluation of antioxidant and free radical scavenging. Dpph free radical scavenging activity of the extracts of.
Among them, the 2,2diphenyl1picrylhydrazyl dpph spectrophotometric method is one of the most widely applied and is appreciated for its reliability. Herein we report the phytochemical analysis and free radical scavenging activity of their sequential extracts. Solvent effects and improvements in the deoxyribose degradation assay for hydroxyl radicalscavenging xican li. Both these assays are essentially dependent upon free radical mechanisms. A dpph free radical scavenging activity 1diphenyl2picrylhydrazylfree radical. This assay uses this character to show herbs free radical scavenging activity. The graduate school, silpakorn university has approved and accredited the thesis title of the role of standardized mangifera indica l. A highthroughput relative 2,2diphenyl1picryhydrazyl dpph radical scavenging capacity rdsc assay was developed and validated in the present study. The results of scavenging effect of tested plant extracts on dpph radical are given in table 1. Freshly prepared dpph solution was taken in test tubes and extracts were added followed by serial dilutions 15. Scavenging activity % x 100 a control super oxide radical scavenging activity super oxide radical o 2. Pegg, in advances in food and nutrition research, 2019.
Onehalf milliliter of the solution was mixed with 0. The color changes from deep purple to pink to yellow after reduction, which can be quantified by its decrease of absorbance at wavelength 518 nm. The dpph assay is a typical offline detection method, where the antioxidant activity is measured colorimetrically. Highthroughput relative dpph radical scavenging capacity assay. Radical scavenging activity increased with increasing. An invitro evaluation s ganapaty 1, vm chandrashekhar 2, hr chitme 1, m lakashmi narsu 3 1 pharmacognosy and phytochemistry division, college of pharmaceutical sciences, andhra university, visakhapatnam 530 003, andhra pradesh, india 2 department of pharmacology, hanagal shri kumareshwar college of pharmacy, bvvs campus. Flavonoids are reported to exhibit various biological activities, including antioxidative and free radical scavenging activities.
Radicalscavenging activity, aceinhibiting capability and. In vitro dpph free radical scavenging assay dpph radical is scavenged by antioxidants through the donation of proton forming the reduced dpph. Measurement of the dpphradical scavenging activity. On the other hand the lowest capacity to reduce dpph was observed in j. The ethanolic extract exhibited higher free radical scavenging effect than the water extract at all tested concentrations. The crude extracts of plants were mixed with 95% methanol to prepare stock solution 1. School of chinese herbal medicine, guangzhou university of chinese medicine, guangzhou, china article info article history. Synthesis of salidroside analogues and their ability of. Different aliquots of the sample extracts were added to 3 ml of a 6 x 10 5 moll dpph methanolic solution. Free radical scavenging ability of the extracts was tested by dpph radical scavenging assay as described by blois and desmarchelier et al. Phytochemical analysis and free radical scavenging. Glycosaminoglycans, for example chondroitin sulfate, heparan.
The dpph assay measures the free radical scavenging capacity of the extracts as described previously. Free radical scavenging in vitro and biological activity of diphenyl diselenideloaded nanocapsules. An assessment of the potential of proline to scavenge free radicals was made in a couple of in vitro assay systems, namely graft copolymerization and autooxidation of pyrogallol. The inhibitory percentage of dpph was calculated according to the following equation.
In the horac hydroxyl radical averting capacity assay, antioxidants can be. For each compound, five concentrations were employed and the percentage of remaining dpph was determined after 30 min at 37. Invitro antioxidant and free radical scavenging activity of. The scavenging rate of abts was enhanced at the improved concentration of rsp. Dpph is a common abbreviation for the organic chemical compound 2,2diphenyl1picrylhydrazyl. This method was developed by blois with the viewpoint to determine the antioxidant activity in a like manner by using a stable free radical. Dpph radical scavenging capacity of phenolic extracts from.
The hopping increases additionally the values of the parameter. Several methods have been developed to assess the radical scavenging activity. Quantitative determination of free radical scavenging. The free radical scavenging activity of all the extracts was evaluated by 1, 1diphenyl2picrylhydrazyl dpph according to the previously reported method by shen et al. Of the extracts of the medicinal plants, species showed % scavengingactivityfrom to %,whileremainingintherange of to %. The assay was carried out in buffered medium methanol. Determination of the free radical scavenging potential of the samples scavenging activity of dpph radical. Dpph free radical scavenging activity of the extracts of the. The hydrogen atom donating ability of the plant extractives was determined by the decolorization of methanol solution of 2,2diphenyl1picrylhydrazyl dpph. In vitro antioxidant properties, free radicals scavenging. Antioxidant enzymes and dpphradical scavenging activity in. The antiradical activity of caffeic acid 1, dihydrocaffeic acid 5, and their corresponding nalkyl esters was evaluated by using the 2,2diphenyl1picrylhydrazyl radical dpph method. The aim of this work is to study and compare the antioxidant properties and phenolic contents of aqueous leaf extracts of juniperus thurifera, juniperus oxycedrus, juniperus phoenicea, and tetraclinis articulata from morocco.
The differences between methods conditions and their evaluation are presented. Abts radicalscavenging activity abts is a stable organic free radical, which can directly re. A perusal of the publications in the recent past table 1 shows that various research groups have used widely different protocols which differed in. The extracts of seeds, leaves, and stem bark of jamun have also been observed to be free radical scavengers in the dpph and other scavenging assays. It is a darkcolored crystalline powder composed of stable freeradical molecules. I read a few journals on dpph assay for vegetable, however they did not state how much to add and the concentration in. Evaluation of antioxidant and free radical scavenging capacities of polyphenolics from pods of caesalpinia pulcherrima.
The odd electron of nitrogen atom in dpph is reduced by receiving a hydrogen. After 20 min incubation at room temperature, read the absorbance at 517 nm. Dpph free radical scavenging activity of phenolics and. Dpph 1,1diphenyl2picrylhydrazyl is considered as a stable radical because. The percentage of antioxidant activity aa% of each substance was assessed by dpph free radical assay. The differences between the free radical scavenging activity of laboratory and production. Free radical scavenging in vitro and biological activity. Free radical scavenging activity of ethanolic extracts. Statistical analysis test of significance of the data obtained from the free radical scavenging activity of plant extract and bha using ttest paired two sample for means showed that the difference between the free radical scavenging activities of plant extract and bha on the natural ros used, was significant p proteases activities, and phenolics composition of bark extracts from 21 medicinal plants muhammadasamraza, 1,2 durreshahwar, 1 andtaniakhan 1 center for natural product drug development, department of chemistry, government college university, lahore, pakistan. A modified dpph assay was conducted to evaluate free radical scavenging activity using methanol extract of h. Dpph radical assay the radical scavenging capacities of each ethanolic extract in different concentrations were estimated according to the method of brandwillians et al. Synthesis of salidroside analogues and their ability of dpph. Free radical scavenging and total antioxidant capacity of.
Scavenging of dpph radical is the basis of the popular dpph antioxidant assay alma et al. Received 17 october 2012 received in revised form 30 march 20 accepted 8 may 20 available online 23 may. Invitro antioxidant and free radical scavenging activity. Antioxidant activity by dpph assay of potential solutions.
Solvent effects and improvements in the deoxyribose. Rapid highthroughput assay to assess scavenging capacity. Antioxidant activities of the extracts were evaluated by 2,2diphenyl1picrylhydrazyl dpph free radicalscavenging ability, trolox equivalent. Stock solution of the whole plant extracts was prepared to the concentration. The antioxidant activity of the extracts was measured on the basis of the scavenging activity of the stable 1, 1 diphenyl 2picrylhyorazyl dpph free radical according to the method described by brandwilliams et al22 with slight modifications.
Three milliliters of each of the diluted extracts were put in the test tube and 1 ml of a methanol solution of dpph 0. The measurement of the dpph radical scavenging activity was performed according to methodology described by brandwilliams et al. Genesis and development of dpph method of antioxidant assay. Several flavonoids obtained from barley leaves, soybean and some medicinal plants, silybum marianum, sophorae flos, cinnamon, ephedrae herba and scutellariae radix, were tested for their dpph 1,1diphenyl2. Dpph 1,1diphenyl2picrylhydrazyl radical scavenging.
Dpph has two major applications, both in laboratory research. Dpph radical scavenging assay free radical scavenging ability of the extracts was tested by dpph radical scavenging assay as described by blois 23 and desmarchelier et al. Using dpph radical scavenging assay to measure antioxidants in vegetable oil. During the different stages of the brewing process the free radical scavenging activity is changed. Radicalscavenging activity and ferric reducing ability of. The survey of the methods for determination of free radical scavenging activity by dpph has been done. The dpph radical scavenging activity s% was calculated using the following equation. Dpph free radical scavenging capacity of legume extracts was evaluated according to the method of chen and ho 11 as modified by xu and chang 10. Free radical scavenging activity of crude extracts and 4. The samples were reacted with the stable dpph radical in an ethanol solution. The antioxidant activity of the memq was evaluated by the phosphomolybdenum method according to the procedure of prieto et al. In vitro antioxidant and free radical scavenging activity of. Antioxidant activity by dpph assay of potential solutions to. It is a darkcolored crystalline powder composed of stable free radical molecules.
Dpph free radical scavenging assay the free radical scavenging capacity of the crude extracts of the medicinal plants was determined using dpph as described by reena et al 2012. Gnidia glauca and dioscorea bulbifera are traditional medicinal plants that can be considered as sources of natural antioxidants. Using dpph radical scavenging assay to measure antioxidants. Dpph 1,1diphenyl2picrylhydrazyl radical scavenging activity of flavonoids obtained from some medicinal plants. Stock solution of the whole plant extracts was prepared to the concentration of 1 mgml. Radical scavenging capacity of n2mercapto2methylpropionyllcysteine 455 components of the extracellular matrix include glycosaminoglycans, collagen and other fibrous proteins, glycoproteins, and specialized polysaccharides that form a jellylike or watery ground substance. Feb 25, 2011 dpph assay is considered a valid accurate, easy and economic method to evaluate radical scavenging activity of antioxidants, since the radical compound is stable and need not be generated.
Different solvent extracts petroleum ether, chloroform and hydroethanolic of the formulation was tested for its dpph assay, super oxide radical, hydroxyl radical and nitric oxide. Research article radical scavenging, proteases activities. In the final step, 12 were converted to w in 92% yield and x in 90%. The extract i mgml showed marked protection up to 66. Highthroughput relative dpph radical scavenging capacity. Hydrogen atom transfer is an essential step in the termination of radical chain reactions involved in lipid oxidation. This rdsc assay is easy to perform and has acceptable accuracy 90. Free radicalscavenging capacity, antioxidant activity and. Statistical analysis test of significance of the data obtained from the free radical scavenging activity of plant extract and bha using ttest paired two sample for means showed that the difference between the free radical scavenging activities of plant extract and bha on the natural ros used, was significant p radical scavenging potential of p. Antioxidant activity dpph assay radical scavenging increased with antioxidant.
The assay is based on the measurement of the scavenging capacity of antioxidants towards it. Free radical scavenging activity, total phenolic content. Rapid assay highthroughput assay scavenging capacity index abstract a new microplateadapted dpph rapid assay was developed to assess the antioxidant capacity of pure compounds and foods. Procedure for free radical scavenging activity the ability of the extracts to annihilate the dpph radical 1,1diphenil2picrylhydrazyl was investigated by the method described by blois, 1958.
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